As shown in Fig. Further analysis and quantification were done as previously described 5. Multiple and essential Sp1 binding sites in the promoter for transforming growth factor-beta type I receptor. The different responses of these two PDGF receptors were likely due to the innate differences in available adaptor molecules that transmit their signals in different cell types Seventy-two hours later cells were harvested for further experiments.

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Received Apr 9; Accepted Aug Cells were harvested for Western blot WB. J Exp Med S1; supplemental material for this article is available online at the Journal of American Physiology Gastrointestinal and Liver Physiology website.

Immunoprecipitation and Western blot. Focal adhesion assembly in myofibroblasts fosters a microenvironment that promotes tumor growth. Data are representative of multiple repeats. Seventy-two hours later cells were harvested for further experiments.

Data are representative of multiple experiments with consistent results. Cell nuclei were counterstained by Hoechst dye blue.


Kang at the Hormel Institute. Support Center Support Center.

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F759 Y, Massague J. These conditioned media were used as stimulants in cell proliferation assays and chemoattractants in Boyden chamber assays. Data represent 3 repeats. STEM is an extensively used antibody for detection of human-derived cells in mice, owing to its specific reaction with a human cytoplasmic antigen. Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover.

These 2 proteins colocalized at the plasma membrane arrowheads and in intracellular vesicles arrows. More than six representative cells in each group were selected for quantification and statistical analysis.

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Mechanisms of TGF-beta signaling from cell membrane to the nucleus. No conflicts of interest, financial or otherwise, are declared by the authors. Semin Oncol 38Suppl 1: Densitometry of WB is shown on the right. Open in a separate window. Mechanism of activation of the TGF-beta receptor. Mechanisms of hepatic fibrogenesis. Author information Article notes Copyright and License information Disclaimer. Cell nuclei were counterstained by Hoechst dye.


WB was carried out as we previously described 5. Role of platelet-derived growth factors in physiology and medicine.

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Furthermore, our study is also highly relevant to other diseases that are associated with myofibroblastic activation, including liver, lung, and renal fibrosis. Multiple and t759 Sp1 binding sites in the promoter for transforming growth factor-beta type I receptor. Transforming growth factor beta activation of c-Abl is independent of receptor internalization and regulated by phosphatidylinositol 3-kinase and PAK2 in mesenchymal cultures. Platelet-derived growth factor receptor signaling activates pericyte-myofibroblast transition in obstructive and post-ischemic kidney fibrosis.

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